Part:BBa_K731400:Design
IPTG inducible Cysteine desulfhydrase (CysDes)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1560
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq cassette (BBa_K731300) is in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.
The nucleotide sequence of CysDes Part BBa_K731300 used to create this composite part has been codon optimised before Gene Synthesis by Genescript.
Source
K731300 and K731600
References
(1) Chu L, Burgum A, Kolodrubetz D, and Holt SC. The 46-kilodalton-hemolysin gene from Treponema denticola encodes a novel hemolysin homologous to aminotransferases. Infect Immun 1995 Nov; 63(11) 4448-55.
(2) Awano N, Wada M, Kohdoh A, Oikawa T, Takagi H, and Nakamori S. Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli. Appl Microbiol Biotechnol 2003 Aug; 62(2-3) 239-43.
(3) Wang CL, Maratukulam PD, Lum AM, Clark DS, and Keasling JD. Metabolic engineering of an aerobic sulfate reduction pathway and its application to precipitation of cadmium on the cell surface. Appl Environ Microbiol 2000 Oct; 66(10) 4497-502.